Gradient Method Transfer

Enter your dimensions / particle sizes in the green boxes below.
Existing Method New Method
  Parameter   Parameter New Column Dimensions Adjusted Flow Rate
Column
geometry
Particle Size µm Column
geometry
Particle size µm 1.9 µm
Column length mm Column length mm 50 mm
Column ID mm Column ID mm 2.1 mm
Method
conditions
Flow rate ml/min Method
conditions
Flow Rate 0.55 ml/min ml/min
Injection volume µl Injection volume 0.69 µl 0.69 µl
Pressure Pressure 328 bar 598 bar
System dwell volume ml System dwell volume ml 0.07 ml
Gradient Time %A %B Gradient Time %A %B Time %A %B
Initial Conditions Initial Conditions 0 95 5 0 95 5
Step 2 (initial hold) Step 2 (initial hold) 0 95 5 -0.12 95 5
Step 3 Step 3 0 95 5 0 95 5
Equilibration time 17 min Equilibration time 2 min 1 min
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